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Through this work we have contributed an additional 57 RSVA and RSVB whole virus genomes to GenBank, significantly increasing the total number of available genomes for RSV. In addition, these genomes have considerable geographic diversity. By incorporating additional publically available sequences in our analyses we were able to develop a better understanding of the evolution and global circulation of RSV viruses. We found that the GA2 and GA5 clades for RSVA and the BA clade for RSVB have been the predominant RSV clades circulating globally for at least the past 10 years. Importantly, we did not observe a strong spatial structure in the tree, with evidence that both main RSVA clades co-circulate globally. These findings suggest that the evolution of RSVA and RSVB is not strongly regionalized, although some small clades were only identified in one country (e.g., GA7 in the United States). Further surveillance and sequencing of RSV is required to determine if these minor clades are still circulating in under sampled regions, as well as to know whether certain RSV lineages dominate over sustained time periods within a defined region, or fluctuate year-to-year. Our phylogenies also indicate that previously used genotype classifications may not be monophyletic, and therefore highlight the importance of developing improved nomenclature for RSV.
The game was directed by Tokuro Fujiwara, who previously worked primarily on arcade games such as Ghosts 'n Goblins (1985). Fujiwara toured the film's set to gather inspiration for the game, and the film's director Kiyoshi Kurosawa gave Fujiwara permission to take some liberties with the game's script. Sweet Home was released in December 1989 exclusively in Japan, where it gathered generally favorable reception and was considered better than the film. The game was never localized to western markets, likely because of the game's gruesome imagery and the unpopularity of role-playing games outside Japan.
Sweet Home was released for the Famicom in Japan on 15 December 1989.[14] It was promoted alongside the movie in the film trailer.[12] The game received generally favorable reviews, and many critics believed the game was better than the film.[11] Famitsu provided a score of 28/40.[14] In the 1989 Famitsu Best Hit Game Awards, Sweet Home received a critics' choice award.[15] 153554b96e