The 3 chosen polysorbate (PPS) (P-10, P-20 and P-80) solution concentrations that were used in the studies were determined by the following experiment. In final formulation, the polysorbate stock solution was diluted to a total volume of 75 1 for Cell Sorter run. Using tubes without cells, a serial dilution of the polysorbate solution was made (from 75 1 to 0.01 1).
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