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We aimed to select proteins that are uniquely present in eocytes, with the exception of dikarya (S. cerevisiae, A. thaliana, and O. sativa), and that are preferentially expressed in eocytes relative to all phyla.
InfA-encoding genes are required for the expression of the chloroplast-encoded psaA and psbA genes. Chloroplast-encoded infA genes are lost from a wide range of angiosperm chloroplast genomes. Chloroplast-encoded infA genes present in all angiosperm plastid genomes, including the largest plastid genome of the powderwort (O. sativa) [102]. Therefore, chloroplast-encoded infA genes are probably lost to enhance the nuclear-encoded infA. The chloroplast-encoded infA genes in angiosperm chloroplasts may be remnants of previously lost ancient infA genes in the plastid ancestor of glaucophytes, cyanobacteria, and chloroplasts. The loss event in angiosperms may have occurred independently several times, and most of the species are currently lacking a infA gene in their plastid genomes. In addition, the loss event may have been accelerated by the use of the infA gene for plastid-encoded psaA and psbA expression in angiosperm nuclear genomes.
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